DEOXYRIBONUCLEASES OF MOUSE TISSUES

Characterization of Deoxyribonucleases

Increases in deoxyribonuclease activity assayed at alkaline pH can be observed in poxvirus-infected cells when native or denatured deoxyribonucleic acid (DNA) is used as substrate. The deoxyribonuclease assayable with native DNA as substrate, induced in HeLa cells by cowpoxvirus or vaccinia virus WR, can be separated from the corresponding enzyme present in normal cells by chromatography on die...

Deoxyribonucleases in Streptomyces.

The Deoxyribonucleases of Escherichia coli

Exonuclease I from Escherichiu coli (I) (previously designated as E. coZi phosphodiesterase (2)), shows a high degree of selectivity for denatured or single stranded deoxyribonucleic acid. This enzyme attacks at the 3’-hydroxyl end of a polydeoxyribonucleotide and liberates 5’-mononucleotides in a stepwise manner until the terminal dinucleotide, which is not attacked. Because of these propertie...

Regulation of virus-induced deoxyribonucleases.

The Deoxyribonucleases of Streptococcus Pyogens.

Streptococci of serological groups A and C are known to produce substantial amounts of extracellular deoxyribonuclease (l-4). Evidence that the nuclease activity is probably due to the presence of more than one enzyme was suggested by the finding of Bernheimer (5) that ribonucleic acid from a variety of sources could inhibit to varying degrees the DNase activity of group A streptococci but did ...